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KMID : 0613820070170070905
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Journal of Life Science
2007 Volume.17 No. 7 p.905 ~ p.909
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Adenoviral-Mediated Ref-1 Overexpression Potentiates NO Production in Bradykinin-Stimulated Endothelial Cells
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Song Ju-Dong
Kim Kang-Mi
Lee Sang-Kwon
Kim Jong-Min
Park Young-Chul
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Abstract
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The dual-function protein redox factor-1 (Ref-1) is essential for base excision repair of oxidatively damaged DNA and also governs the activation of many redox-sensitive transcription factors. We examined the role of Ref-1 in regulation of nitric oxide (NO) synthesis employing adenoviral-mediated overexpression of Ref-1 in bradykinin-stimulated endothelial cells. Intracellular NO was detected with the NO-sensitive fluorophore DAF-2. Overexpression of Ref-1 potentiates bradykinin-stimulated NO production in endothelial cells. And, cells infected with AdRef-1 showed higher fluorescence intensity compared with uninfected or AdDl312-infected cells. In parallel with this, overexpression of Ref-1 also stimulated endothelial NO synthase (eNOS) enzyme activity, compared with uninfected or AdDl312-infected cells, in bradykinin-stimulated cells as well as in unstimulated cells. These results suggest that Ref-1 implicates in endothelium-dependent vasorelaxation resulting from NO production in vascular system.
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KEYWORD
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Ref-1, bradykinin, adenoviral gene transfer, NO, endothelial cells
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